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Maxam-Gilbert sequencing
A method of rapid sequencing of DNA molecules developed by Allan Maxam and Walter Gilbert. The technique uses specific chemical reactions to break DNA at specific nucleotides. The DNA is first radiolabeled with 32P at the 5' or 3' end of a chain. Second, the DNA is chemically modified and cleaved at various points along the backbone. Third, the DNA fragments are analysed with polyacrylamide gel electrophoresis.
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