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Dintzis experiment
The method by which it was demonstrated that proteins are synthesised in the N- to C-terminal direction. A labelled amino acid was supplied to a reticulocyte preparation synthesising haemoglobin and the completed globin chains were isolated after a brief incubation. The radioactive products were found to be concentrated in the C-terminus, indicating that this was the last part of the molecule to be formed.
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- Category: Biochemistry; Molecular biology
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