Home > Terms > English, UK (UE) > RNA single-strand conformational polymorphism (rSSCP)
RNA single-strand conformational polymorphism (rSSCP)
A variant of the SSCP technique for detection of mutations in a DNA fragment in which mobility shifts of bands of mutant compared with normal DNA are more pronounced. A phage promoter is added to one of the PCR primers to allow production of an RNA transcript that is then analysed for altered electrophoretic bands on non-denaturing gel electrophoresis.
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