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Chloramphenicol acetyltransferase (CAT) assay
A procedure for evaluation of the regulatory properties of eukaryotic promoter sequences. The CAT gene (which encodes an enzyme found only in bacteria) is used as a 'reporter gene' in that it is fused to a promoter sequence and introduced into a eukaryotic cell, where the ability of the promoter to cause the expression of the CAT gene is monitored by assay of the enzyme's activity; the assay may involve thin-layer chromatographic analysis of the conversion of (14C)chloramphenicol into acetyl (14C)chloramphenicol.
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- Category: Biochemistry; Molecular biology
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