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Dot blotting
A method to estimate the concentration of a polynucleotide or protein solution by spotting it on a sheet of nitrocellulose or nylon, hybridising it with a radiolabelled complementary polynucleotide or antibody, visualising it by autoradiography, then comparing its intensity with that of a similarly treated graded concentration series of the standard polynucleotide or protein.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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