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Fast-atom bombardment mass spectrometry (FAB-MS)
A technique for the analysis of protein sequence and structure. A high energy beam of atoms or ions (Cs or Xe) vapourizes, fragments and ionises a protein solution, the mass spectrum of which gives details of the peptide sequence and post-translational modifications such as N-terminal acylation, glycosylation, phosphorylation and disulphide bridging. Flow FAB is a variant that analyses the continuous liquid stream from a high pressure liquid chromatography or capillary electrophoresis separation.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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