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Random activation of gene expression (RAGE)
A procedure for creating a genome-wide library of cDNAs by use of several insertion vectors, each containing a promoter sequence linked to an exon and followed by an unpaired splice site. Transfection into eukaryotic cells allows the vectors to randomly insert by non-homologous recombination. Where the insertion is upstream from a host cell gene, it activates expression of a transcript that encodes a chimeric protein that containing the vector-encoded sequence and some or all of the host cell protein.
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