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Recognition site

A nucleotide sequence - composed typically of 4, 6 or 8 nucleotides - that is recognised by and to which a restriction endonuclease (restriction enzyme) binds. For type II restriction enzymes (those used in gene-cloning experiments) it is also the sequence within which the enzyme specifically cuts (and their corresponding enzymes methylate) the DNA, i.e., for type II enzymes, the recognition site and the target site are the same sequence. Type I enzymes bind to their recognition site and then cleave the DNA at some more or less random position outside that recognition site.

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