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Home > Terms > English, UK (UE) > Restriction site
Restriction site
The specific nucleotide sequence in DNA that is recognised by a type II restriction endonuclease and within which it makes a double-stranded cut. Restriction sites usually comprise four or six base pairs that typically are palindromic (q.v.),
e.g., 5´GGCC3´
3´CCGG5´
The two strands may be cut either opposite to one another, to create blunt ends, or in a staggered manner, giving sticky ends, depending on the enzyme involved.
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