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Unnatural amino acid mutagenesis
A method for replacement of an amino acid residue of a protein with a non-naturally occurring amino acid. The mRNA that encodes the protein is modified to encode a nonsense suppressor codon. For example, mRNA is modified to use the UAG codon; this mRNA, together with a nonsense suppressor tRNA with the anticodon CUA, and with an unnatural amino acid at its 3'-end, is introduced into an expression system, possibly a Xenopus oocyte.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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