Molecular biology

Molecular biology

Industry: Biology

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Molecular biology

Intron homing

Biology; Biochemistry

The direction of introns, as mobile genetic elements, to sites in DNA. The process is dependent upon proteins that are encoded by open reading frames of the introns: endonucleases in the case of ...

Initiation complex

Biology; Biochemistry

The ribosome charged with mRNA, initiation factors and methionyl-tRNA (in eukaryotes) or N-formylmethionyl-tRNA (in prokaryotes, mitochondria and chloroplasts) that is bound to the initiation codon.

Hydrophobic cluster analysis (HCA)

A method for comparison of amino acid sequences of proteins to detect regions of conformational similarity. Groups of neighbouring hydrophobic amino acid residues are identified in a HCA plot that is ...

Repertoire cloning

Biology; Biochemistry

A method to produce human monoclonal antibodies. mRNAs are isolated from a tissue that generates immunoglobulins (e.g. spleen) and a reverse transcriptase makes cDNA copies of them. The Fd regions, ...

Magnet-assisted subtractive technique (MAST)

Biology; Biochemistry

A method for detection of the tissue specificity of gene expression. Two tissues are compared: a deficient tissue from which single-stranded cDNA (driver cDNA) is prepared and attached to magnetic ...

Diffraction pattern

Biology; Biochemistry

The array of reflections obtained by crystallography. Each reflection indicates an intensity and, by its location, the angle with respect to the incident beam. Because a sharp pattern requires the ...

Homologue cloning

Biology; Biochemistry

A strategy for selection of polynucleotide sequences closely homologous to one that is known by its partial hybridization with a probe designed for the latter; e.g. a labelled probe that is ...