Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A cell that is useful in production of quantities of a monoclonal antibody; a fusion of a myeloma cell and a spleen lymphocyte that, like the myeloma cell, can divide in culture and, like the spleen ...

Structural profile

Biology; Biochemistry

An approach to prediction of similarities in protein conformations by comparison of amino acid sequences. A two- or three-dimensional plot of one or two features, e.g. optimal matching ...

Hypoxanthine/aminopterin/thymidine (HAT) medium

Biology; Biochemistry

A growth medium that is used to isolate monoclonal-antibody-producing cells, i.e. hybrids made from fusion of myeloma cell mutants that lack hypoxanthine-guanine phosphoribosyltransferase, and ...

Homologous recombination

Biology; Biochemistry

The non-symmetrical crossing-over of genes at homologous sequences (e.g. at Alu sequences of introns between different exons of a single gene) which gives rise to two mutant alleles, one a deletion ...

DNA methylation

Biology; Biochemistry

A phenomenon that represses expression of regions of the genome. Transcription is prevented when the DNA is methylated and folded into nucleosomes. Eukaryotic DNA is methylated almost exclusively as ...

Hyperchromic effect

Biology; Biochemistry

The increase in absorbance, frequently measured at 260nm, when a native polynucleotide is denatured. The ratio of absorbance of the native polymer to that of the denatured or hydrolysed material is ...

Pre-initiation complex

Biology; Biochemistry

In replication, transcription or translation, a complex that is potentially composed of template, enzymes and initiation factors but that is incomplete for optimal function due to the absence of one ...