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Maxam-Gilbert method
A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel electrophoresis separates the products according to chain length, and reveals the distance from the radiolabelled 3'- or 5'-end to the chemically modified base.
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- Category: Biochemistry; Molecular biology
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