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Bio-imprinting
The induction of a new catalytic specificity in an enzyme in an organic solvent. An enzyme and a weakly binding non-substrate/non-product (e.g. chymotrypsin and N-acetyl-D-tryptophan) are suddenly precipitated together from an aqueous solution by addition of a miscible organic solvent. The complex reverts to the native conformation and behaviour when redissolved in water, but if redissolved in a suitable organic solvent it will be found to possess a new enzymic activity, e.g. for synthesis of N-acetyl-D-tryptophan ethyl ester from N-acetyl-D-tryptophan and ethanol.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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