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Circularization
A DNA fragment generated by digestion with a single restriction endonuclease will have complementary 5´ and 3´ extensions (sticky ends). If these ends are annealed and ligated, the DNA fragment will have been converted to a covalently-closed circle, or circularized.
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- Part of Speech: noun
- Synonym(s):
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- Industry/Domain: Biotechnology
- Category: Genetic engineering
- Organization: FAO
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