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Minisequencing
A multiplex method for scanning genomic DNA samples for the presence of specific mutations. PCR primers complementary to the mutant and wild-type sequences are immobilised at coded loci on a surface and exposed to genomic DNA samples. Incorporation of a fluorescent marker during PCR amplification will indicate by the presence of fluorescence at a locus, the existence there of the mutant or wild-type template in the genomic DNA to which it had been exposed.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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