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Home > Terms > English, UK (UE) > Random DNA amplification (RDA)
Random DNA amplification (RDA)
A method to produce improved strains, e.g. salt tolerance of a plant, by gene amplification. Random genome fragments are introduced into a vector and grown in a bacterium. The plasmid is then introduced into the original strain at the DNA sequence homologous with the DNA fragment, under conditions that favour tandem repeats. Modified strains are selected first by a resistance marker that was part of the original plasmid, then by cycles of selection under pressure of the desired property, e.g. at high salinity.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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