Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Second law of thermodynamics

Biology; Biochemistry

The relationship of free energy (G), enthalpy (H) and entropy (S) when a system changes from one equilibrium state to another: ΔG=ΔH-TΔS, where T is the absolute temperature. The law provides the ...

RecA-assisted restriction cleavage by endonuclease (RACE cleavage)

A method for cleavage of double-stranded DNA at specific restriction sites. A suitable restriction site is selected, around which the base sequence is known, and an oligonucleotide, up to 50 or 60 ...

Differential genome display

Biology; Biochemistry

A method for identifying candidate genes that may determine significant differences between closely related species whose genomes have been mapped, e.g. between a pathogen and a closely related ...

Reporter gene

Biology; Biochemistry

A gene that is coupled with a gene of primary interest when they are inserted together into a cell. The reporter gene allows identification of those few cells that have been successfully transformed ...

Deletion mapping

Biology; Biochemistry

The assignment of the relative positions of deletion mutations along chromosomes. In the first phase, many individuals are studies for the presence of cytogenetic markers and later phases use ...

Antisense

Biology; Biochemistry

Descriptive of an endogenous or semi-synthetically produced oligoribonucleotide complementary to mRNA and capable of base-pairing and annealing with mRNA to prevent translation; or of an ...

AT queue

Biology; Biochemistry

A model that accounts for chromosomal banding. The microscopic appearance of chromosomes during mitosis (metaphase) displays chromomeres and interchromomeres, i.e. G bands (Giemsa stain) and R bands ...