Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

In evolution theory, phyletic gradualism, the proposition that evolution occurs at a slow and generally constant rate involving a large fraction of an entire species that gradually separates from the ...

Cysteine scanning

Biology; Biochemistry

The use of site-directed mutagenesis to determine, for residues of a complex, usually an uncrystallized protein, their proximity to other residues of the protein, their mileiu (extracellular, ...

Phage-display

Biology; Biochemistry

A method for incorporation, into the DNA that encodes a phage coat protein, of a polypeptide, where it is available for a selection procedure; especially for production and selection of antibody ...

Altered-self hypothesis

Biology; Biochemistry

The proposal that helper T-cells recognise a foreign material, e.g. a viral antigen, when it is presented on the surface of an antigen-presenting cell as a complex with the major histocompatibility ...

Aggregation

Biology; Biochemistry

The formation of higher-molecular-mass species due to non-covalent adherence of smaller species. Especially for proteins, aggregation is a form of denaturation in which non-polar surfaces of ...

Cassette mutagenesis

Biology; Biochemistry

A method to determine the limits of tolerance of a protein to amino acid substitution. A cloned gene is mutated by synthesis of short segments of the gene with random base substitutions, insertion of ...

Minisatellite variant repeat-specific priming PCR (MVR-PCR)

Biology; Biochemistry

A method for characterization of DNA samples of individuals for forensic purposes, based on sequence variations within the repeating units of variable number tandem repeats (VNTRs). PCR primers are ...