Molecular biology

Molecular biology

Industry: Biology

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Molecular biology

Binary system

Biology; Biochemistry

In transgenic research, an approach to control expression of one transgene by a second, each initially established in its own pedigree. By crossing the two lines, doubly transgenic animals are ...

RNA ligase reverse transcription PCR (RL-RT-PCR)

A procedure for amplification of minute amounts of an unknown RNA (or DNA) sequence. The RNA fragment is ligated at both ends to single-stranded palindromic oligodeoxynucleotide that provides a site ...

Ultraviolet (UV) spectroscopy

Biology; Biochemistry

A technique which measures absorption of electromagnetic radiation at wavelengths shorter than the visible spectrum, i.e. below 400 nm. In biological samples, UV spectroscopy detects aromatic ...

Photosynthetic unit (PSU)

Biology; Biochemistry

The light-harvesting complex and reaction center. Light is absorbed at 875 nm by proteins with caretenoid prosthetic groups, and the energy is transferred to photosystem II (PSII) of the reaction ...

Hanging-drop technique

Biology; Biochemistry

A method for slowly changing concentrations in a protein solution in an attempt to prepare crystals. A small volume of protein solution (of the order of 5 μl) on a microscope coverslip is sealed ...

Bacteriophage

Biology; Biochemistry

A virus that infects bacteria. Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells. Lytic phage, e.g. the T series ...

Lipopolysaccharide (LPS)

Biology; Biochemistry

A constituent of the outer membrane of Gram-negative bacteria; composed of an outward-directed and highly variable oligosaccharide (the O-antigen), which is responsible for the antigenicity of the ...