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Dixon plot

A graphical method for determination of the type of enzyme inhibition and the dissociation constant (Ki) for an enzyme-inhibitor complex. The effect on the enzymic rate (v) is determined at two or more substrate concentrations, and over a range of inhibitor concentrations (I). In a plot of 1/v against I, data for each substrate concentration fall on straight lines that intersect at I=-Ki and 1/v=1/Vmax (competitive inhibition), or that intersect on the abscissa (1/v=0) at I=-Ki (non-competitive inhibition). For uncompetitive inhibition, the lines are parallel.

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