Affinity chromatography

Affinity chromatography

The purification of a substance by means of its affinity for another substance immobilised on a solid support; for example, an antigen can be purified by affinity chromatography on a column of antigen-specific antibody molecules covalently linked to beads.

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Part of Speech: noun
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Industry/Domain: Biology
Category: Immunology
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affinity chromatography

Biology; Immunology

The purification of a substance by means of its affinity for another substance immobilized on a solid support; for example, an antigen can be purified by affinity chromatography ...

affinity chromatography

Chemistry; Analytical chemistry

A chromatographic technique that utilizes the ability of biological molecules to bend to certain ligands specifically and reversibly; used in protein biochemistry.

affinity chromatography

Biotechnology; Genetic engineering

A method for separating molecules by exploiting their ability to bind specifically to other molecules. There are several types of biological affinity chromatography. A biological ...

affinity chromatography

Biology; Biochemistry; Molecular biology

The separation of soluble macromolecules by use of a stationary phase that is designed to interact specifically with, and thus retard the elution of, the desired material; e.g. a ...

affinity chromatography

Medical; Health care; Medicine

A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry.

immuno-affinity chromatography

Biotechnology; Genetic engineering

A purification technique in which an antibody is bound to a matrix and is subsequently used to bind and separate a protein from a complex mixture.

unresponsiveness

Biology; Immunology

Inability to respond to antigenic stimulus. Unresponsiveness may be specific for a particular antigen, or broadly nonspecific as a result of damage to the entire immune system, ...

V(D)J recombination

Biology; Immunology

Mechanism for generating antigen-specific receptors of T and B cells; it involves the joining of V, D, and J gene segments mediated by the enzyme complex V(D)J recombinase, and ...

vaccination

Biology; Immunology

Any protective immunization against a pathogen. Originally referred to immunization against smallpox with the less virulent cowpox (vaccinia) virus.

variable (V) regions

Biology; Immunology

The N-terminal portion of an Ig or TCR which contains the antigen-binding region of the molecule; V regions are formed by the recombination of V(D) and J gene segments.

virion

Biology; Immunology

A complete virus particle.

virus

Biology; Immunology

An organism comprising a protein coat and DNA or RNA genome; it requires a host cell for replication.

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