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Nuclear run-off assay
A method to determine which genes in a population of cells are expressed at a given time. Nascent RNA transcripts are radiolabelled with (32P)NTPs as they are elongated in isolated nuclei (new transcripts are not initiated in these isolated nuclei). To determine if a specific gene is expressed, the radiolabelled run-off transcripts are used as hybridization probes with DNA from recombinant plasmids that contain the gene of interest.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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