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Home > Terms > English, UK (UE) > Restriction-site-generating PCR (RG-PCR)
Restriction-site-generating PCR (RG-PCR)
A method for detection of a specific mutation that does not itself cause a unique restriction fragment length polymorphism, because it neither creates nor removes a known restriction site. A new restriction site is created by the introduction of a deliberately mismatched PCR primer, the 5'-end of which is designed to hybridise to the mutant template and the 3'-end of which encodes a new restriction site.
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- Part of Speech: noun
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- Industry/Domain: Biology
- Category: Biochemistry; Molecular biology
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