Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Catalytic rate constant (kcat)

Biology; Biochemistry

The first-order rate constant that describes the rate-limiting step in enzyme catalysis, usually the conversion of the enzyme-substrate complex into the enzyme-product complex; the maximal velocity ...

Magic spot nucleotides

Biology; Biochemistry

Guanine nucleotide derivatives (ppGpp and pppGpp) that mediate the cessation of production of rRNA in the absence of an essential amino acid, a phenomenon present in stringent bacteria but absent ...

Polarisation

Biology; Biochemistry

A measure of the mobility of a fluorophore: P=(I

Fast-atom bombardment mass spectrometry (FAB-MS)

Biology; Biochemistry

A technique for the analysis of protein sequence and structure. A high energy beam of atoms or ions (Cs or Xe) vapourizes, fragments and ionises a protein solution, the mass spectrum of which gives ...

Pulse labelling

Biology; Biochemistry

In metabolic studies, the exposure of a biological system (e.g. a tissue slice, a cell culture, mitochondria) to a radiolabelled metabolite over a relatively short time, in order to subsequently ...

Fast-atom bombardment (FAB) mapping

Biology; Biochemistry

A technique for comparison of recombinant with natural proteins or with expected structures. A protein is cleaved by a specific enzymic (e.g. trypsin) or chemical (e.g. CNBr) method and the product ...

Sedimentation-velocity ultracentrifugation

Biology; Biochemistry

A technique that measures the rate of movement of a dissolved polymer under the force exerted upon it in a centrifuge, to assist in evaluation of molecular mass, axial ratio and other hydrodynamic ...