Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

Signal sequence; the N-terminal portion of a secretory or membrane protein that assists it across the membrane of the rough endoplasmic reticulum, where it is synthesized, but which is cleaved from ...

Nuclear run-off assay

Biology; Biochemistry

A method to determine which genes in a population of cells are expressed at a given time. Nascent RNA transcripts are radiolabelled with ( 32 P)NTPs as they are elongated in isolated nuclei (new ...

Topology/packing diagram

Biology; Biochemistry

A schematic representation of secondary structure of a protein in which α-helix is rendered as a circle, β-sheet as a rectangle and connecting sequences as lines. The circles and rectangles are ...

Sequence-tagged connector (STC)

Biology; Biochemistry

An approximately 500 bp sequence at each end of a bacterial artificial chromosome (BAC). If the human genome is cleaved into 300000 fragments, of an average length of 150 kb, there would be 600000 ...

Rolling circle replication

Biology; Biochemistry

Mechanisms of DNA replication of small circular genomes, such as some viruses and plasmids, in which the template generates multiple tandem copies. One strand is nicked and serves as a primer for ...

Equilibrium sedimentation ultracentrifugation

Biology; Biochemistry

A technique for evaluation of the molecular mass of a polymer by determination of the extent of sedimentation at which the force exerted upon a macromolecule in a centrifuge is balanced by its ...

Rolling transcription

Biology; Biochemistry

The synthesis of RNA from a circular DNA template. Some circular DNAs, even without a promoter sequence and without assistance of a primer, may be transcribed to form concatenated circular RNA. If ...