Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

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Biology; Biochemistry

A measure of the location of an amino acid residue in a protein molecule; the number of α-carbons, excluding those of the adjacent residues in the linear sequence, within an 8 Ã radius of the ...

A technique for the separation of macromolecules from smaller molecules by placing them within a semi-permeable membrane, such as Cellophane, separating them from a large volume of water. Only the ...

Cot unit

Biology; Biochemistry

A compound unit used in measurement of the complexity of a DNA preparation. The rate of annealing of DNA is controlled by the second-order rate of nucleation, expressed as C o /C=1+k 2 C o t, ...

Displacement chromatography

Biology; Biochemistry

A column chromatographic method for separation and concentration of components of a solution. After adsorption of the sample to be separated on to the resin in a column, all bound molecules are ...

Monodisperse

Biology; Biochemistry

Descriptive of a population of polymers in which all individual molecules have the same covalent structure and molecular size. It is contrasted with polydisperse, descriptive of a population of ...

Ligation-independent cloning of PCR products (LIC-PCR)

Biology; Biochemistry

A method for cloning complex mixtures of PCR products to achieve a library of recombinant clones. PCR-amplified sequences, e.g. those bracketed by Alu sequences, are generated with primers that ...

Denaturing gradient gel electrophoresis (DGGE technique)

Biology; Biochemistry

A method for detection of single base substitutions in DNA fragments. Putative mutant and normal double-stranded DNA fragments are applied along one edge of an agarose gel slab that contains a ...