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Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

Western blotting

Biology; Biochemistry

A technique to detect specific proteins. A mixture of proteins is separated by polyacrylamide-gel electrophoresis, blotted on to a plastic sheet (e.g. of nitrocellulose) and then exposed to a ...

Immunoblotting

Biology; Biochemistry

A technique to detect specific proteins. A mixture of proteins is separated by polyacrylamide-gel electrophoresis, blotted on to a plastic sheet (e.g. of nitrocellulose) and then exposed to a ...

Domain

Biology; Biochemistry

A region of a protein that has a stable secondary and tertiary structure that can fold independently of other domains; often connected to other domains, of the same protein if any, by short sequences ...

Southern blotting

Biology; Biochemistry

A technique for detection of specific DNA fragments. Restriction fragments are separated by polyacrylamide-gel electrophoresis, blotted on to a plastic sheet (e.g. of nitrocellulose) and detected by ...

Proofreading

Biology; Biochemistry

In DNA replication, the 3'-to-5' exonuclease activity of a polymerase that removes incorrectly paired bases, also referred to as editing; in protein synthesis, the hydolysis of an incorrectly paired ...

Editing

Biology; Biochemistry

In DNA replication, the 3'-to-5' exonuclease activity of a polymerase that removes incorrectly paired bases, also referred to as editing; in protein synthesis, the hydolysis of an incorrectly paired ...

Oligonucleotide array

Biology; Biochemistry

In biotechnology, a component of a device for screening genomic or cDNA for mutations, polymorphisms or gene expression. A chip is a small (a few centimetres on each side) standardised glass or other ...

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