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Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

Chip

Biology; Biochemistry

In biotechnology, a component of a device for screening genomic or cDNA for mutations, polymorphisms or gene expression. A chip is a small (a few centimetres on each side) standardised glass or other ...

Ion trap

Biology; Biochemistry

A mass spectrograph in which vaporised ions are confined by an AC electric field. As the radio-frequency voltage that confines the ion is changed, the ion will escape the trap at a voltage ...

Southwestern blotting

Biology; Biochemistry

A technique for identification of DNA-binding proteins and regions of DNA that bind proteins which combines aspects of Southern and western blotting techniques. A nuclear extract is separated by ...

Chemical cleavage method

Biology; Biochemistry

A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel ...

Maxam-Gilbert method

Biology; Biochemistry

A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel ...

Conjugation

Biology; Biochemistry

In organic chemistry, the interaction of double or triple bonds or aromatic groups, separated by a single bond, which delocalizes their non-bonding electrons; e.g. -CH=CH-CH=CH-, ...

Supercoiling

Biology; Biochemistry

The torsional stress placed on double-stranded DNA, maintained for example by interaction with proteins or by DNA that is circular, and accommodated by a twist imposed on the duplex. A left-handed ...

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