Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Instructive theory

Biology; Biochemistry

A now discarded hypothesis that explains antibody specificity by the folding of an antibody structure, which has the potential for many specificities, to the unique surface of a particular antigen.

The process of assembling the DNA sequences that encode an immunoglobulin or a T-cell receptor (TCR), the cell-surface protein that recognises an antigen. The process excises polynucleotide sequences ...

Meselson-Radding model

Biology; Biochemistry

A conceptualization of general genetic recombination during meiosis of newly replicated double-stranded DNA. An endonuclease nicks one strand of one daughter duplex; the new 5'-end dissociates and ...

Hydrophobic moment

Biology; Biochemistry

A measure of the amphipathicity of an Â¿-helix. Each amino acid side chain is assigned a value, positive or negative, according to its hydrophobicity. These values are used to weight vectors for each ...

Electroblotting

Biology; Biochemistry

An electrophoretic technique for transfer of a protein or nucleic acid from a slab gel, or similar unstable two-dimensional matrix, to a sheet of stable material such as nitrocellulose or ...

Thiocyanate degradation

Biology; Biochemistry

A method for analysis of peptide structure by repetitive sequential degradation from the C-terminus. The free a-carboxyl group is activated with acetic anhydride and reacted with thiocyanate, and the ...

Radioimmunoassay (RIA)

Biology; Biochemistry

A competitive binding assay that depends upon displacement of a radiolabelled ligand from an antibody by the non-labelled standard or test sample, followed by separation of the labelled ligand into ...