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Biochemistry
The scientific study of the chemistry of living matter.
Industry: Biology; Biotechnology
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Biochemistry
Molecular chaperone
Biology; Biochemistry
Enzymes and other cellular protein complexes that ensure the proper folding of nascent or improperly folded proteins. There are two classes of chaperone: the heat shock protein (hsp) 70 family and ...
Single-strand conformational polymorphism (SSCP)
Biology; Biochemistry
A technique for detection of mutations in a defined DNA sequence. A labelled PCR-amplified segment of DNA is heat-denatured then quenched at 08C, and the resultant single-stranded polynucleotides are ...
Chaperone machine
Biology; Biochemistry
A multi-component system that ensures the proper folding of nascent proteins or their intracellular transport following synthesis; components include chaperones (eukaryotes) or chaperonins (from ...
Retrotransposon
Biology; Biochemistry
A mobile genetic element composed of repetitive DNA sequences that flank open reading frames, including one that codes for a reverse transcriptase. In contrast to transposons, which in prokaryotes ...
Signal recognition particle (SRP)
Biology; Biochemistry
In the synthesis of secretory, endoplasmic reticulum (ER), Golgi apparatus, lysosomal and integral membrane proteins, a ribonucleoprotein that recognises a signal sequence and prevents further ...
Molecular ruler
Biology; Biochemistry
A method for measurement of distances on a molecular scale; the estimation of distances between a fluorescence energy donor and receptor, evaluated from the efficiency of resonance energy transfer, ...
Representation
Biology; Biochemistry
A portion of the genome with lowered complexity (e.g. by cleavage with an infrequent cutting restriction nuclease, ligated to oligonucleotide adapters so it can then be amplified by PCR), but that ...