Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

An indication of the position of an amino acid residue within a proposed folding of a globular protein; the number of α-carbons within a 14Ã (1.4nm) radius, which varies from about 10 for a residue ...

RNA chaperone

Biology; Biochemistry

A putative RNA-binding protein that assists RNA in attainment of its biologically active conformation. Non-specific RNA-binding proteins are proposed to prevent RNA becoming temporarily trapped in ...

Proton motive force

Biology; Biochemistry

The free energy, expressed in volts, represented by a pH gradient across the membrane of a vesicle due to the concentration gradient plus the membrane potential. This energy is used by coupling the ...

Reverse transcriptase-PCR (RT-PCR)

Biology; Biochemistry

Also known as RNA phenotyping, RNA-PCR or message amplification phenotyping; a method for amplification of a specific mRNA by prior use of reverse transcriptase to form a cDNA, then use of PCR to ...

Pool

Biology; Biochemistry

The aggregate of metabolites that are in a functional equilibrium in a cell or an organism, e.g. cytosolic hexose phosphates, mitochondrial tricarboxylic acid cycle intermediates, liver glycogen.

Ribosome display

Biology; Biochemistry

An alternative to phage display as a method for selection of a protein with optimised binding properties, which is based on the principles of polysome display. A cell-free expression system ...

Polysome display

Biology; Biochemistry

An alternative to phage display as a method for selection of a protein with optimised binding properties. A cell-free bacterial expression system transcribes a DNA library and the resulting mRNAs are ...