Molecular biology

Molecular biology

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Molecular biology

Michaelis constant (Km)

Biology; Biochemistry

Given by (k -1 +k 2 )/k 1 ; the sum of the first-order rate constants for the productive and non-productive breakdown of the Michaelis complex divided by the second-order rate constant for its ...

Archaic nomenclature for a protein that is sparingly soluble in water, but is soluble in dilute salt solutions. Euglobulins do not dissolve in salt-free water, whereas pseudoglobulins are soluble in ...

Flame photometry

Biology; Biochemistry

An analytical technique to quantify small amounts of some elements, especially alkali metals, in solution; the solution is vapourized in a flame and the intensity of the characteristic spectral line ...

Quiescent affinity label

Biology; Biochemistry

A reagent that reacts specifically with the active site of the enzyme for which it was designed. It bears a generally reactive chemical group, such as a chloromethane, and substrate-like moieties ...

Catalytic rate constant (kcat)

Biology; Biochemistry

The first-order rate constant that describes the rate-limiting step in enzyme catalysis, usually the conversion of the enzyme-substrate complex into the enzyme-product complex; the maximal velocity ...

Magic spot nucleotides

Biology; Biochemistry

Guanine nucleotide derivatives (ppGpp and pppGpp) that mediate the cessation of production of rRNA in the absence of an essential amino acid, a phenomenon present in stringent bacteria but absent ...