Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A variation of the PCR technique, similar to ligation-mediated PCR, that is applied to double-stranded DNA fragments for which the sequence at only one end of the gene is known. The technique allows ...

Fluorescence in situ hybridization (FISH)

Biology; Biochemistry

A method for visualisation of a genetic marker on a chromosome by use of a fluorescent labelled polynucleotide probe that hybridises to and indicates the locus of a gene on a chromosome during ...

Double-sieve editing

Biology; Biochemistry

A proposed mechanism that accounts for the high selectivity of some enzymes, especially the amino acid activating enzymes. An initial screening sorts substrates by size - those too large cannot enter ...

Tier

Biology; Biochemistry

The cohort of chains of a branched-chain polysaccharide that all have the same relationship to the branch points. The outer tier of glycogen is all those glucosyl units that are attached in α1→4 ...

Leader sequence

Biology; Biochemistry

Signal sequence; the N-terminal portion of a secretory or membrane protein that assists it across the membrane of the rough endoplasmic reticulum, where it is synthesized, but which is cleaved from ...

Nuclear run-off assay

Biology; Biochemistry

A method to determine which genes in a population of cells are expressed at a given time. Nascent RNA transcripts are radiolabelled with ( 32 P)NTPs as they are elongated in isolated nuclei (new ...

Topology/packing diagram

Biology; Biochemistry

A schematic representation of secondary structure of a protein in which α-helix is rendered as a circle, β-sheet as a rectangle and connecting sequences as lines. The circles and rectangles are ...