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Density-gradient centrifugation

A technique to separate and/or characterise a macromolecule by high-speed centrifugation in a density gradient formed by the concentration gradient of a solute such as CsCl or sucrose. The macromolecule sediments until it reaches the zone of its own density. Also known as isopycnic centrifugation or zonal centrifugation.

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Related terms

density gradient centrifugation
Chemistry; Analytical chemistry

Separation of particles according to density by employing a gradient of varying densities; at equilibrium each particle settles in the gradient at a point equal to its density.

sucrose density gradient centrifugation
Biotechnology; Genetic engineering

A procedure used to fractionate mRNAs or DNA fragments on the basis of size.

equilibrium density gradient centrifugation
Biotechnology; Genetic engineering

A procedure used to separate macro-molecules based on their density (mass per unit volume).

velocity density gradient centrifugation
Biotechnology; Genetic engineering

A procedure used to separate macromolecules based on their rate of movement through a density gradient.

density gradient centrifugation
Biotechnology; Genetic engineering

High-speed centrifugation in which molecules "float" at a point where their density equals that in a gradient of caesium chloride or sucrose. The density gradient may either be ...

density gradient centrifugation
Medical; Health care; Medicine

Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density.

calcareous
Biology; Biochemistry

The body parts of animals which contain calcium as shells, bones and exoskeleton and they protect the animals, are called as calcareous.

humus
Biology; Biochemistry

Humus is a dark material in the soil that is formed from decaying plants and leaves.

red blood cell
Biology; Biochemistry

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rotten
Biology; Biochemistry

Anything that has gone bad; smelly; decayed; evil; unpleasant which is likely to break due to rot is called as rotten.

aerobic
Biology; Biochemistry

Needing or using oxygen to live.Living things that can exist only in environments that contain oxygen are aerobic.

anderogen
Biology; Biochemistry

One of the hormones that controls the physical development in males. Testosterone is an androgen.

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