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Sequence-tagged site (STS)

A landmark in the base sequence of a genome used for placing partial sequences in correct relation to each other; especially as a strategy for co-ordination of the efforts of many laboratories in sequencing the human genome. Originally, the use of defined PCR primers allowed the examination of DNA for the ability to generate a 200-500 bp amplicon which indicated the presence of the STS. With the growth of banked genome data, electronic PCR (e-PCR), the automated search of databases for STSs as DNA sequences of the desired size and orientation and bracketed by sites complementary to the defining primers, is possible as a non-laboratory, entirely computer based procedure

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Related terms

sequence-tagged site (STS)
Biotechnology; Genetic engineering

Short, unique DNA sequence (usually 200 to 500 bp) that, by being able to be amplified by PCR, is uniquely "tagged" to the site on the chromosome from which it was amplified.

sequence tagged site (STS)
Archaeology; Human evolution

A short segment of DNA that defines a unique position in the human genome; an STS is usually detected by the polymerase chain reaction.

sequence-tagged site (STS)
Agriculture; Rice science

Region of DNA on a chromosome with known sequences used as a signpost for molecular gene mapping approaches.

sequence tagged site (STS)
Biology; Genome

Short (200 to 500 base pairs) DNA sequence that has a single occurrence in the human genome and whose location and base sequence are known. Detectable by polymerase chain ...

sequence-tagged site (STS)
Biology; Biochemistry; Molecular biology

A landmark in the base sequence of a genome used for placing partial sequences in correct relation to each other; especially as a strategy for co-ordination of the efforts of many ...

sequence tagged site (STS)
Biology; Biochemistry; Molecular biology

Any site in a chromosome or genome that is identified by a known unique DNA sequence. STSs can be used to form genetic maps by standard mapping procedures.

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