Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

The use of a transposon to isolate a gene whose product may be unknown. Mutants in which a transposon inserts into and disrupts the function of a gene are selected by phenotype. A DNA fragment that ...

Cotton effect

Biology; Biochemistry

A feature of some optical rotatory dispersion spectra that is usefully correlated with conformational and structural features of a molecule. A sharp peak in optical rotation followed by a deep trough ...

Electrophoretic mobility shift assay (EMSA)

Biology; Biochemistry

A method to identify by function a band on a polyacrylamide gel. Electrophoresis is performed on a mixture of proteins before and after treatment with a strong ligand that is specific to one ...

Gel shift assay

Biology; Biochemistry

A method to identify by function a band on a polyacrylamide gel. Electrophoresis is performed on a mixture of proteins before and after treatment with a strong ligand that is specific to one ...

Random DNA amplification (RDA)

Biology; Biochemistry

A method to produce improved strains, e.g. salt tolerance of a plant, by gene amplification. Random genome fragments are introduced into a vector and grown in a bacterium. The plasmid is then ...

Regulation

Biology; Biochemistry

The phenomenon of adjustment of metabolite flux, e.g. glucose that enters the anaerobic glycolysis pathway and exits as lactate, whatever its molecular mechanism. It reflects for each step of the ...

Gibbs-Donnan effect

Biology; Biochemistry

The unequal distribution of a diffusible ion across a semi-permeable membrane when an impermeable electrolyte, such as a protein, is also present on one side; e.g. a solution of an anionic protein ...