Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

One of the alternative DNA sequences that may occur at a given chromosomal locus. An individual with identical alleles at this locus on both of his homologous chromosomes is a homozygote; one with ...

Designability

Biology; Biochemistry

A hypothetical property of a protein folding motif; its relative thermodynamic and evolutionary stabilities, i.e. resistance to denaturation and changes in its primary sequence, respectively. A motif ...

Stokes' Law

Biology; Biochemistry

A theoretically derived relationship between the viscosity coefficient, η, the frictional coefficient, f, and the radius of a macromolecule (the Stokes radius) r: f=6πηr. This calculated f may be ...

Protein truncation test (PTT)

Biology; Biochemistry

In vitro synthesised protein assay; a procedure for detection of mutations that result in a protein of significantly different size, e.g. by early termination, insertion, change in reading frame or ...

Immuno-PCR

Biology; Biochemistry

A very sensitive method for detection of antigens. The antigen is immobilised in wells of a microtitre plate, exposed to its specific monoclonal antibody, then to a chimaeric protein that consists of ...

Cloverleaf

Biology; Biochemistry

The formalised pattern assumed by a tRNA molecule viewed in two dimensions that shows the regions of internal complementarity that allow the polynucleotide to fold back upon itself into base-paired ...

Homologous ligation

Biology; Biochemistry

A method for fusion of two oligonucleotides, independently of any restriction site they share. A region flanking the desired ligation site on one fragment is introduced into the second fragment by ...