Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

Recruitment of the exons that code for a functional domain of one protein in the evolution of a gene for a new protein, to produce hybrids with recognisable motifs, such as kringles and metal-binding ...

Chloramphenicol acetyltransferase (CAT) assay

Biology; Biochemistry

A procedure for evaluation of the regulatory properties of eukaryotic promoter sequences. The CAT gene (which encodes an enzyme found only in bacteria) is used as a 'reporter gene' in that it is ...

Chou-Fasman analysis

Biology; Biochemistry

A method for prediction of the secondary structure of a protein from its primary structure. A survey of proteins of known secondary and tertiary structure allows an empirical classification of amino ...

Restriction endonculease fingerprinting (REF)

Biology; Biochemistry

A method for analysis of large restriction fragments of DNA with a high degree of confidence of finding mutations. The large fragment is separately digested with several cocktails (preferably five) ...

TUNEL technique

Biology; Biochemistry

The method of terminal deoxy-transferase (TdT) and dUTP assisted nick end labelling for detection of apoptosis in single cells. The method takes advantage of the fact that the DNA of apoptotic cells ...

Indirect end labelling

Biology; Biochemistry

A method to locate, between restriction sites, binding sites on DNA, e.g. the positions of nucleosomes on a minichromosome. The DNA protected by binding proteins is cleaved in susceptible, i.e. ...

Zipper-type mechanism

Biology; Biochemistry

A mechanism for proteolytic degradation of a native protein, in which cleavage of the first peptide bond in each molecule is much faster than subsequent cleavages. The result is that every native ...