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Molecular biology
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Molecular biology
Alpha (α)-configuration
Biology; Biochemistry
In steroid chemistry, the orientation of substituents below the plane of the ring system, i.e. on the side opposite the angular methyl groups at C-10 and C-13, which have the β-configuration. ...
Tandem mass spectrometry (tandem MS; MS/MS)
Biology; Biochemistry
An adaptation of MS for the analysis of molecular structure; an initial separation by MS generates a peak, i.e. a molecular ion or large fragment, that is further fragmented by gas-phase collision, ...
Protein
Biology; Biochemistry
A linear polymer of a-amino acids held together by peptide bonds. A protein may include other components attached covalently (e.g. carbohydrate, phosphate, fatty acid, biotin) or non-covalently (e.g. ...
Thiotemplate mechanism
Biology; Biochemistry
A non-ribosomal, non-RNA-dependent mechanism for the synthesis of bacterial cyclic oligopeptide antibiotics (e.g. gramicidin S) in which the amino acid residues are first transferred from amino acid ...
Random oligonucleotide mutagenesis
Biology; Biochemistry
A technique to mutate all bases of a gene. DNA synthesis proceeds from an assortment of oligonucleotide primers synthesised by substitution at each point of the sequence of all three incorrect ...
Sulfane
Biology; Biochemistry
A persulfide, a compound of the type RS 2 H or RS 2 - ; the group includes biosynthetic Âreactive sulfur compounds, which may be precursors of ÂinorganicÂsulfur, e.g. non-haem iron proteins, ...
Saturation mutagenesis
Biology; Biochemistry
A technique to mutate all bases of a gene. DNA synthesis proceeds from an assortment of oligonucleotide primers synthesised by substitution at each point of the sequence of all three incorrect ...