Molecular biology

Molecular biology

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Molecular biology

Methylation-specific PCR (M-PCR)

Biology; Biochemistry

A technique for mapping methylation patterns of CpG islands of genomic DNA. Genomic DNA is first treated with sodium bisulphite (NaHSO 3 ) to convert cystosine residues, but not 5-methylcytosine ...

Carboxyl-terminal analysis

Biology; Biochemistry

A chemical method for determination of C-terminal residues of proteins by cleavage by hydrazinolysis, which generates amino acid hydrazides of all amino acids except the C-terminal one; also an ...

Residualizing label

Biology; Biochemistry

A moiety attached to a circulating protein in order to subsequently detect by histological means the site of the protein's degradation in a multicellular organism. A reporter group, such as a ...

Heteroduplex analysis

Biology; Biochemistry

A method for detection of single base substitutions in DNA fragments. The PCR-amplified putatively mutated DNA fragment is mixed with homologous normal DNA fragments; any mutated sequence is much ...

Mismatch analysis

Biology; Biochemistry

A method for detection of single base substitutions in DNA fragments. The PCR-amplified putatively mutated DNA fragment is mixed with homologous normal DNA fragments; any mutated sequence is much ...

Mechanism-based inhibitor

Biology; Biochemistry

An enzyme inhibitor that differs from the classical substrate or product analogue. One category comprises transition-state analogues that do not form covalent links to the enzymes but bind very ...

Meselson-Stahl experiment

Biology; Biochemistry

A demonstration of semi-conservative DNA replication. E. coli grown on 15 N salts for several generations produce labelled DNA that is more dense than normal DNA, as judged by density gradient ...