Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A stack of flattened vesicles that functions in the post-translational processing and sorting of proteins. The Golgi receives proteins from the rough endoplasmic reticulum (RER) and directs them to ...

Luciferin

Biology; Biochemistry

A potentially chemiluminescent substrate; the enzymic reaction of an enzyme (a luciferase) converts it to an excited state that decays with emission of visible radiation; e.g. the benzothiazole of ...

Prion

Biology; Biochemistry

A proteinaceous infectious particle which causes one of a number of non-inflammatory, slowly-developing degenerative neurological diseases which are characterised by extracellular plaques, notably ...

Boomerang DNA amplification (BDA)

Biology; Biochemistry

A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a ...

DUTP system mutagenesis

Biology; Biochemistry

A method to construct a site-specific mutated gene and to select it away from the wild-type. A strain of E. coli with only weak dUTPase activity has high levels of dUTP and consequently incorporates ...

Prenyl

Biology; Biochemistry

A chemical group composed of multiples of the branched, unsaturated, 5-carbon isoprene unit, e.g. geranyl (10 carbons), farnesyl (15 carbons), geranylgeranyl (20 carbons). It is sometimes attached to ...

Hydrogen exchange

Biology; Biochemistry

A method for evaluating secondary and tertiary structure of a polypeptide in solution. The amide hydrogens of the polypeptide backbone slowly exchange with water hydrogens, unless they are ...