Molecular biology

Molecular biology

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Molecular biology

Partition chromatography

Biology; Biochemistry

A technique for separation of molecules based on their differing ratios of solubility in two immiscible solvent phases. A stationary phase is coated or impregnated with one solvent phase and the ...

Iontophoresis

Biology; Biochemistry

The transport of ions across a semi-permeable membrane under the influence of an electric charge. As ions are driven across the membrane by the electric field, there is also the accompanying ...

Periodic acid-Schiff (PAS) stain

Biology; Biochemistry

A reagent for staining of carbohydrate in polyacrylamide gels or histological preparations; periodic acid first oxidises vicinal hydroxy groups to generate aldehydes that can then condense with ...

Nick-translation

Biology; Biochemistry

A technique often used for introduction of 32 P into a DNA duplex involving cleavage of a phosphodiester bond of one strand, excision of the newly exposed nucleoside 5'-phosphate residue, and ...

The internal movement possible in some proteins, in which domains show little internal movement but move relative to each other by flexion at the hinges that connect them, e.g. the wagging movement ...

Autophagocytosis

Biology; Biochemistry

The response of a cell to stress in which it forms an autophagosome, an organelle that contains parts of the cell's own cytoplasm. Later this will fuse with a lysosome to form an autolysosome; all ...

Carbon cycle

Biology; Biochemistry

The movement of carbon atoms through different chemical forms and locations, from dissolved CO 2 in equilibrium with atmospheric CO 2 through plant carbohydrate, fats and proteins of plants and ...