Molecular biology

Molecular biology

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Molecular biology

PCR-based differential scanning

Biology; Biochemistry

A method for amplification of a heterogeneous population of cDNAs preparatory to creation of a library. Total mRNA is copied first using an oligo(dT) primer to obtain one copy, then that primer is ...

Synthetase

Biology; Biochemistry

An enzyme, regardless of its mechanism, that couples two substrates by a carbon-carbon, carbon-oxygen, carbon-sulphur or carbon-nitrogen bond that is driven by the hydrolysis of a phosphoanhydride ...

Trans-recognition

Biology; Biochemistry

A refinement of the N-end rule; the determination of the rate of degradation of a cellular multimeric protein composed of different kinds of subunits by amino acid residues of a subunit other than ...

RNA virus

Biology; Biochemistry

A virus with an RNA genome that may be either an mRNA, (+)-RNA, or its complement, (-)-RNA. Class 1 contains (+)-RNA; class 2, (-)-RNA, which is the template for an RNA-dependent RNA polymerase; ...

Minisequencing

Biology; Biochemistry

A multiplex method for scanning genomic DNA samples for the presence of specific mutations. PCR primers complementary to the mutant and wild-type sequences are immobilised at coded loci on a surface ...

Strand displacement amplification (SDA)

Biology; Biochemistry

A technique for amplification of DNA sequences. The source DNA is denatured and hybridised to phosphorothioated primers. HincII nicks its target site on the unmodified strand of the heteroduplex and ...

Linker-scanner mutation

Biology; Biochemistry

A method to assess the importance of various regions of a protein to its function by introduction of a dipeptide sequence into random sites of the protein. A plasmid that contains a structural gene ...