Molecular biology

Molecular biology

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Molecular biology

Restriction endonculease fingerprinting (REF)

Biology; Biochemistry

A method for analysis of large restriction fragments of DNA with a high degree of confidence of finding mutations. The large fragment is separately digested with several cocktails (preferably five) ...

TUNEL technique

Biology; Biochemistry

The method of terminal deoxy-transferase (TdT) and dUTP assisted nick end labelling for detection of apoptosis in single cells. The method takes advantage of the fact that the DNA of apoptotic cells ...

Indirect end labelling

Biology; Biochemistry

A method to locate, between restriction sites, binding sites on DNA, e.g. the positions of nucleosomes on a minichromosome. The DNA protected by binding proteins is cleaved in susceptible, i.e. ...

Zipper-type mechanism

Biology; Biochemistry

A mechanism for proteolytic degradation of a native protein, in which cleavage of the first peptide bond in each molecule is much faster than subsequent cleavages. The result is that every native ...

Allele

Biology; Biochemistry

One of the alternative DNA sequences that may occur at a given chromosomal locus. An individual with identical alleles at this locus on both of his homologous chromosomes is a homozygote; one with ...

Designability

Biology; Biochemistry

A hypothetical property of a protein folding motif; its relative thermodynamic and evolutionary stabilities, i.e. resistance to denaturation and changes in its primary sequence, respectively. A motif ...

Stokes' Law

Biology; Biochemistry

A theoretically derived relationship between the viscosity coefficient, η, the frictional coefficient, f, and the radius of a macromolecule (the Stokes radius) r: f=6πηr. This calculated f may be ...