Molecular biology

Molecular biology

Industry: Biology

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Molecular biology

Displacement chromatography

Biology; Biochemistry

A column chromatographic method for separation and concentration of components of a solution. After adsorption of the sample to be separated on to the resin in a column, all bound molecules are ...

Monodisperse

Biology; Biochemistry

Descriptive of a population of polymers in which all individual molecules have the same covalent structure and molecular size. It is contrasted with polydisperse, descriptive of a population of ...

Ligation-independent cloning of PCR products (LIC-PCR)

Biology; Biochemistry

A method for cloning complex mixtures of PCR products to achieve a library of recombinant clones. PCR-amplified sequences, e.g. those bracketed by Alu sequences, are generated with primers that ...

Denaturing gradient gel electrophoresis (DGGE technique)

Biology; Biochemistry

A method for detection of single base substitutions in DNA fragments. Putative mutant and normal double-stranded DNA fragments are applied along one edge of an agarose gel slab that contains a ...

A 10 to 200 nm protein-, cholesterol- and sphingolipid-rich microdomain of a membrane (e.g. plasma, endoplasmic reticulum, mitochondrial). These rafts are more ordered and thus denser than the lipid ...

Coincidence painting (chromosome painting)

Biology; Biochemistry

A method for production and utilisation of fluorophore-labelled oligonucleotides to identify very localised regions of chromosomes. The method of degenerate oligonucleotide primed PCR (DOP-PCR) ...

Double adaptor method

Biology; Biochemistry

CDNA concatamer sequencing; a method for increasing the efficiency of sequencing cDNA library subclones cDNA fragments are cleaved with a restriction nuclease and randomly religated into larger DNA ...