Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

In vitro synthesised protein assay; a procedure for detection of mutations that result in a protein of significantly different size, e.g. by early termination, insertion, change in reading frame or ...

Immuno-PCR

Biology; Biochemistry

A very sensitive method for detection of antigens. The antigen is immobilised in wells of a microtitre plate, exposed to its specific monoclonal antibody, then to a chimaeric protein that consists of ...

Cloverleaf

Biology; Biochemistry

The formalised pattern assumed by a tRNA molecule viewed in two dimensions that shows the regions of internal complementarity that allow the polynucleotide to fold back upon itself into base-paired ...

Homologous ligation

Biology; Biochemistry

A method for fusion of two oligonucleotides, independently of any restriction site they share. A region flanking the desired ligation site on one fragment is introduced into the second fragment by ...

Contact number

Biology; Biochemistry

A measure of the location of an amino acid residue in a protein molecule; the number of α-carbons, excluding those of the adjacent residues in the linear sequence, within an 8 Ã radius of the ...

Dialysis

Biology; Biochemistry

A technique for the separation of macromolecules from smaller molecules by placing them within a semi-permeable membrane, such as Cellophane, separating them from a large volume of water. Only the ...

Cot unit

Biology; Biochemistry

A compound unit used in measurement of the complexity of a DNA preparation. The rate of annealing of DNA is controlled by the second-order rate of nucleation, expressed as C o /C=1+k 2 C o t, ...