Molecular biology

Molecular biology

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Molecular biology

Proton motive Q cycle

Biology; Biochemistry

The cyclic reduction and oxidation of cytochromes b and c 1 and an iron-sulphur protein, which comprise the cytochrome bc 1 complex. In each cycle one ubiquinol is oxidised to ubiquinone, two ...

Plastoquinone/plastoquinol

Biology; Biochemistry

Plastoquinone (PQ) is a quinone molecule involved in the electron transport chain in the light-dependent reactions of photosynthesis. Plastoquinone is reduced (accepts two protons (H+) from the ...

Conservative site-specific recombination (CSSR)

The inversion or excision of a DNA sequence or the integration of a foreign DNA sequence into a homologous genomic site. These all require homologous recombination at the sites of inversion, excision ...

Motif

Biology; Biochemistry

A recurring pattern of protein folding, e.g. a homeobox, a zinc finger. A higher order of protein structure is a module which consists of several motifs and units of secondary structure; in ...

Shuffling

Biology; Biochemistry

Recruitment of the exons that code for a functional domain of one protein in the evolution of a gene for a new protein, to produce hybrids with recognisable motifs, such as kringles and metal-binding ...

Chloramphenicol acetyltransferase (CAT) assay

Biology; Biochemistry

A procedure for evaluation of the regulatory properties of eukaryotic promoter sequences. The CAT gene (which encodes an enzyme found only in bacteria) is used as a 'reporter gene' in that it is ...

Chou-Fasman analysis

Biology; Biochemistry

A method for prediction of the secondary structure of a protein from its primary structure. A survey of proteins of known secondary and tertiary structure allows an empirical classification of amino ...