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Molecular biology
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Molecular biology
Pulsed-field gel electrophoresis (PFGE)
Biology; Biochemistry
A technique for separation of chromosomes and very large DNA molecules, usually for purposes of genetic mapping; alternating pulses of electricity directed at 1208 across the plane of an agarose gel ...
Phospholipid-transfer protein
Biology; Biochemistry
A lipoprotein of the intracellular membranes of eukaryotic cells that can exchange its phospholipid or sterol for membrane components, and can shuttle lipids between these membranes, e.g. between the ...
Cytoskeleton
Biology; Biochemistry
The network of relatively rigid structures within a cell that give it shape and provide a framework against which intracellular movement may take place; includes microtubules, intermediate filaments, ...
Phosphoroclastic split
Biology; Biochemistry
A cleavage in which the elements of phosphate are inserted across a carbon-carbon bond, e.g. the phosphoroclastic reaction of pyruvate, in which pyruvate and orthophosphate are oxidised by a ...
Cluster analysis
Biology; Biochemistry
In genomics, the discovery of genes that are similarly regulated. Statistical analyses are applied to the DNA microarray experiments to identify genes, the expressions of which respond in the same ...
Hirt lysate
Biology; Biochemistry
A preparation from which low-molecular-mass extrachromosomal DNA may be isolated; made by rendering insoluble the higher-molecular-mass DNA and other cellular structures by treatment of cells with a ...
Contact system
Biology; Biochemistry
The cascade of proteolytic activations of blood factors that is initiated by autoactivation of factor XII when it is adsorbed to a surface, e.g. that of a bacterium, and results in uncontrolled ...