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Molecular biology
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Molecular biology
Asymmetrical PCR
Biology; Biochemistry
A protocol for generation of single-stranded DNA. Unequal amounts of primers are used, so that the first PCR cycles generate equal amounts of each strand of the template but later cycles, which have ...
Asymmetrical PCR
Biology; Biochemistry
A protocol for generation of single-stranded DNA. Unequal amounts of primers are used, so that the first PCR cycles generate equal amounts of each strand of the template but later cycles, which have ...
Truncation
Biology; Biochemistry
Elimination of the N- or C-terminal portion of a protein by proteolysis or manipulation of the structural gene, or premature termination of protein elongation due to the presence of a termination ...
Truncation
Biology; Biochemistry
Elimination of the N- or C-terminal portion of a protein by proteolysis or manipulation of the structural gene, or premature termination of protein elongation due to the presence of a termination ...
Oxidative decarboxylation
Biology; Biochemistry
Removal of carbon dioxide from a carboxylic acid that is facilitated by oxidation of the α-carbon (α-oxidative decarboxylation, e.g. the pyruvate dehydrogenase reaction) or the β-carbon (β-oxidative ...
Procollagen suicide
Biology; Biochemistry
The magnified effect of a mutation in the propeptide of one collagen precursor molecule on the structure of secreted collagen. The phenomenon results from the disruption of fibril assembly when a ...
Reverse turn
Biology; Biochemistry
A secondary structure of proteins in which the polypeptide backbone turns sharply on itself in as few as three residues, and is stabilised by hydrogen bonds between the amide hydrogens and carbonyl ...